Product Name | Rel (Phospho-Ser503) Antibody |
Host Species | Rabbit |
Clonality | Polyclonal |
Purification | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
Applications | IF |
Reactivity | Hu |
Specificity | The antibody detects endogenous level of Rel only when phosphorylated at serine 503. |
Immunogen | Peptide-KLH. Peptide sequence around phosphorylation site of serine 503 (T-S-S(p)-D-S) derived from Human Rel. |
Target Name | Rel |
Alternative Name | C-Rel |
Modification | Phospho-Ser503 |
SDS-PAGE MW | 78kd |
Accession No | Q04864; NP_002899.1 |
Concentration | 1.0mg/ml |
Formulation | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Storage | Store at -20°C for long term preservation (recommended). Store at 4°C for short term use. |
Application
Immunofluorescence: 1:100~1:200
Background
The REL gene encodes c-Rel, a transcription factor that is a member of the Rel/NFKB family, which also includes RELA (MIM 164014), RELB (604758), NFKB1 (MIM 164011), and NFKB2 (MIM 164012). These proteins are related through a highly conserved N-terminal region termed the ‘Rel domain,’ which is responsible for DNA binding, dimerization, nuclear localization, and binding to the NFKB inhibitor (MIM 164008) (Belguise and Sonenshein, 2007 (PubMed 18037997)).
Baeuerle, P.A. and Henkel, T. (1994) Annu Rev Immunol 12, 141-79. Baeuerle, P.A. and Baltimore, D. (1996) Cell 87, 13-20. Haskill, S. et al. (1991) Cell 65, 1281-9. Thompson, J.E. et al. (1995) Cell 80, 573-82.
产品名称 人脂联素ELISA试剂盒
测定类型 固相夹心ELISA
格式 96孔板
测定长度 4 .5小时
样品体积 血清,血浆,细胞培养基,100ul
检测方法 比色
灵敏度 20 pg / mL
测定范围 65.5-4000 pg /毫升
反应性 人类
交叉反应性 没有可用相关分子中观察到的交叉反应性
基因名称 ADIPOQ; ACDC;ACRP30;ADIPQTL1; ADPN;APM-1;APM1;GBP28
基因ID 9370
Swissprot ID Q15848
复苏
样品类型 | 平均回收率 | 范围 % |
血清 | 94 | 83-104 |
等离子体 | 91 | 81-102 |
细胞培养基 | 96 | 85-107 |
线性度
样品类型 | 稀释 | 预期百分比 |
血清 | 1:2 | 94 |
1:4 | 92 | |
1:8 | 95 | |
等离子体 | 1:2 | 93 |
1:4 | 95 | |
1:8 | 97 | |
细胞培养基 | 1:2 | 92 |
1:4 | 98 | |
1:8 | 91 |
标准曲线: 人脂联素ELISA试剂盒
•预涂的96孔板微孔板
•洗涤缓冲液
•终止溶液
•分析稀释液
•标准品
•生物素化的检测抗体
•链霉亲和素偶联的HRP
•TMB一步底物
1.按照手册中的说明准备所有试剂,样品和标准品。
2.向每个孔中添加100 µl标准液或样品。
3.在室温或O / N在4°C下孵育2.5小时。
4.洗涤并向每个孔中加入100 µl准备好的生物素抗体。
5.在室温下孵育1小时。
6.洗涤并向每个孔中加入100 µl准备好的链霉亲和素溶液。
7.在室温下孵育45分钟。
8.洗涤并向每个孔中添加100 µl TMB一步底物试剂。
9.在室温孵育30分钟。
10.向每个孔中添加50 µl终止溶液。
11.在450 nm / 570 nm处读取。
脂联素,也称为Acrp30,是脂肪细胞衍生的蛋白质,对代谢和炎症具有广泛的旁分泌和内分泌作用。它促进脂肪细胞分化,脂肪酸分解代谢和胰岛素敏感性,并且与肥胖症,2型糖尿病和动脉粥样硬化形成负相关。脂联素缔合成70 kDa非共价连接的三聚体,该三聚体被二硫键连接成中等分子量的六聚体。
从发货之日起,整个套件可在-20°C下最多保存1年。避免重复冻融循环。该试剂盒可在4°C下最多保存6个月。对于长时间存储,建议在-80°C下存储。
人脂联素ELISA试剂盒仅供研究使用。