Product Name | NFκB-p105(Phospho-Ser893) Antibody |
Host Species | Rabbit |
Clonality | Polyclonal |
Purification | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
Applications | IHC IF |
Reactivity | Hu Ms Rt |
Specificity | The antibody detects endogenous level of total NFkB-p105 protein. |
Immunogen | Peptide-KLH. Peptide sequence around aa.891~895 (A-S-S-P-V) derived from Human NFκB-p105. |
Target Name | NFκB-p105 |
Alternative Name | KBF1; NF-kB1; NFkappaB |
Modification | Phospho-Ser893 |
SDS-PAGE MW | 120kd |
Accession No | P19838; NP_001158884.1 |
Concentration | 1.0mg/ml |
Formulation | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Storage | Store at -20°C. |
Application
Background
Background
NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively.
产品名称 人脂联素ELISA试剂盒
测定类型 固相夹心ELISA
格式 96孔板
测定长度 4 .5小时
样品体积 血清,血浆,细胞培养基,100ul
检测方法 比色
灵敏度 20 pg / mL
测定范围 65.5-4000 pg /毫升
反应性 人类
交叉反应性 没有可用相关分子中观察到的交叉反应性
基因名称 ADIPOQ; ACDC;ACRP30;ADIPQTL1; ADPN;APM-1;APM1;GBP28
基因ID 9370
Swissprot ID Q15848
复苏
样品类型 | 平均回收率 | 范围 % |
血清 | 94 | 83-104 |
等离子体 | 91 | 81-102 |
细胞培养基 | 96 | 85-107 |
线性度
样品类型 | 稀释 | 预期百分比 |
血清 | 1:2 | 94 |
1:4 | 92 | |
1:8 | 95 | |
等离子体 | 1:2 | 93 |
1:4 | 95 | |
1:8 | 97 | |
细胞培养基 | 1:2 | 92 |
1:4 | 98 | |
1:8 | 91 |
标准曲线: 人脂联素ELISA试剂盒
•预涂的96孔板微孔板
•洗涤缓冲液
•终止溶液
•分析稀释液
•标准品
•生物素化的检测抗体
•链霉亲和素偶联的HRP
•TMB一步底物
1.按照手册中的说明准备所有试剂,样品和标准品。
2.向每个孔中添加100 µl标准液或样品。
3.在室温或O / N在4°C下孵育2.5小时。
4.洗涤并向每个孔中加入100 µl准备好的生物素抗体。
5.在室温下孵育1小时。
6.洗涤并向每个孔中加入100 µl准备好的链霉亲和素溶液。
7.在室温下孵育45分钟。
8.洗涤并向每个孔中添加100 µl TMB一步底物试剂。
9.在室温孵育30分钟。
10.向每个孔中添加50 µl终止溶液。
11.在450 nm / 570 nm处读取。
脂联素,也称为Acrp30,是脂肪细胞衍生的蛋白质,对代谢和炎症具有广泛的旁分泌和内分泌作用。它促进脂肪细胞分化,脂肪酸分解代谢和胰岛素敏感性,并且与肥胖症,2型糖尿病和动脉粥样硬化形成负相关。脂联素缔合成70 kDa非共价连接的三聚体,该三聚体被二硫键连接成中等分子量的六聚体。
从发货之日起,整个套件可在-20°C下最多保存1年。避免重复冻融循环。该试剂盒可在4°C下最多保存6个月。对于长时间存储,建议在-80°C下存储。
人脂联素ELISA试剂盒仅供研究使用。